PhD Researcher to Investigate Single-Cell Biophysics in Early Metastasis

To unravel the role of viscoelasticity and adhesion strength in cancer cells using fluid force microscopy

Job description

Mac4Me (Macrophage Targets for Metastatic Treatment) is a pan-European Marie Skłodowska-Curie Doctoral Network (DC) with 18 PhD candidates aimed at understanding the specific tumour cell-immune host interactions at the metastatic site to identify new immune targets. Mac4Me will use animal-free organ-on-chip (OoC) systems to recapitulate early liver, bone and brain metastasis of cancer developing across our life, such as neuroblastoma, breast and prostate cancer. Data from these preclinical models will be integrated with real-world clinical data using AI approaches to identify new immune targets. Mac4Me offers a unique and innovative research training programme comprising both scientific knowledge and transferable skills and integrating participatory science principles as a starting point.

The PhD candidate at TU Delft will focus on investigating the biophysics of single cells in early cancer metastatic conditions. The goal is to quantify shape, size, viscoelasticity and adhesion strength of individual cancer and immune cells to discover potential biophysical disease markers. Experiments will be performed using AFM and FluidFM technology. Suitable analytical models should be developed to analyze the data and interpret the results in collaboration with other partners in the network.

Job requirements

• You have a master degree in Physics, Engineering Physics, Bionanosciences, or Micro/Nano Engineering. Preferably, you have experience in single-cell analysis, atomic force microscopy (AFM) / spectroscopy, microfluidics, statistical data analyis, biophysics, finite element method simulations, analytical modeling and microfabrication.
• As part of your PhD thesis, you will focus on doing topography, viscoelastic and adhesion strength mesurements of single cells using microfluidic AFM probes on a bioAFM instrument.
• You will write scripts to automatically analyse the data (opportunity to use machine learning), build analytical and FEM models to understand the biophysics of metastatic cancer cells in various co-culture environments.
• You will closely collaborate with PhD research students at Erasmus medical center, Technical university in Milan, and Technical university in Dresden.
• As part of your research you will have opportunity to visit collaborators for 3 - 6 months.
• You will be trained to use our high-tech laboratory with advanced microscopy / spectroscopy methods, micro-nano manufacturing, and characterization equipment.

TU Delft (Delft University of Technology)

Delft University of Technology is built on strong foundations. As creators of the world-famous Dutch waterworks and pioneers in biotech, TU Delft is a top international university combining science, engineering and design. It delivers world class results in education, research and innovation to address challenges in the areas of energy, climate, mobility, health and digital society. For generations, our engineers have proven to be entrepreneurial problem-solvers, both in business and in a social context.

At TU Delft we embrace diversity as one of our core values and we actively engage to be a university where you feel at home and can flourish. We value different perspectives and qualities. We believe this makes our work more innovative, the TU Delft community more vibrant and the world more just. Together, we imagine, invent and create solutions using technology to have a positive impact on a global scale. That is why we invite you to apply. Your application will receive fair consideration.

Challenge. Change. Impact!

Faculty Mechanical Engineering

From chip to ship. From machine to human being. From idea to solution. Driven by a deep-rooted desire to understand our environment and discover its underlying mechanisms, research and education at the ME faculty focusses on fundamental understanding, design, production including application and product improvement, materials, processes and (mechanical) systems.

ME is a dynamic and innovative faculty with high-tech lab facilities and international reach. It’s a large faculty but also versatile, so we can often make unique connections by combining different disciplines. This is reflected in ME’s outstanding, state-of-the-art education, which trains students to become responsible and socially engaged engineers and scientists. We translate our knowledge and insights into solutions to societal issues, contributing to a sustainable society and to the development of prosperity and well-being. That is what unites us in pioneering research, inspiring education and (inter)national cooperation.

Conditions of employment

As per transnational mobility rule of Marie-Curie Doctoral networks, the applicant must not have resided or carried out their main activity (work or studies) in The Netherlands for more than 12 months prior to the appointment date for the position at TU Delft.

Doctoral candidates will be offered a 4-year period of employment in principle, but in the form of 2 employment contracts. An initial 1,5 year contract with an official go/no go progress assessment within 15 months. Followed by an additional contract for the remaining 2,5 years assuming everything goes well and performance requirements are met.

Salary and benefits are in accordance with the Collective Labour Agreement for Dutch Universities, increasing from € 2872 per month in the first year to € 3670 in the fourth year. As a PhD candidate you will be enrolled in the TU Delft Graduate School. The TU Delft Graduate School provides an inspiring research environment with an excellent team of supervisors, academic staff and a mentor. The Doctoral Education Programme is aimed at developing your transferable, discipline-related and research skills.

The TU Delft offers a customisable compensation package, discounts on health insurance, and a monthly work costs contribution. Flexible work schedules can be arranged.